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- Primary Antibodies ›
- Apolipoprotein B Antibodies
Proteintech
View all (39) Apolipoprotein B antibodies
Cite APOB Polyclonal Antibody
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APOB Antibody (20578-1-AP) in WB
Mouse liver and Human plasma were subjected to SDS PAGE followed by western blot with (Product # 20578-1-AP) APOB antibody at dilution of 1:1,500 incubated at room temperature for 1.5 hours. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
20578-1-AP
Applications
Tested Dilution
Publications
Western Blot(WB)
1:500-1:1,000
View 25 publications25 publications
Immunohistochemistry (IHC)
-
View 1 publication1 publication
Immunohistochemistry (Paraffin) (IHC (P))
1:20-1:200
-
Immunocytochemistry(ICC/IF)
1:20-1:200
View 3 publications3 publications
Product Specifications
Species Reactivity
Human,Mouse
Published species
Mouse
Host/Isotype
Rabbit/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide
Conjugate
Unconjugated
Form
Liquid
Concentration
0.18 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody recognizes both of ApoB-48 and ApoB-100.
Target Information
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL) with a molecular mass of approximately 260-500 kDa. Apolipoprotein B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation. Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearance of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. MDA-oxidized LDL appear to be different from LDL oxidized by other means. Apolipoprotein B is mostly synthesized in the liver, and is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L. Apolipoprotein B occurs in plasma as two main isoforms, apoB-48 and apoB-100. The intestinal and the hepatic forms of Apolipoprotein B are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in the Apolipoprotein B gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective Apolipoprotein B, diseases affecting plasma cholesterol and Apolipoprotein B levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:Apo B-100; Apo B100; apo-B; ApoB 100; ApoB 48; apolipo b; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B 100; Apolipoprotein B 48; Apolipoprotein B-100; Apolipoprotein B100; apolipoprotein B46; apolipoprotein B47; apolipoprotein B48; apolipoprotein B49; mCG_129875; MGC176318
UniProt ID:(Human) P04114, (Mouse) E9Q414
Entrez Gene ID:(Human) 338, (Mouse) 238055
Molecular Function: apolipoprotein
Function(s)
retinoid metabolic processin utero embryonic developmenttriglyceride mobilizationreceptor-mediated endocytosisspermatogenesisnervous system developmentcholesterol metabolic processfertilizationresponse to virusresponse to carbohydratepost-embryonic developmentresponse to selenium ionpositive regulation of gene expressionpositive regulation of macrophage derived foam cell differentiationpositive regulation of lipid storagepositive regulation of cholesterol storagetriglyceride catabolic processcholesterol transportsperm motilityresponse to lipopolysaccharidecholesterol effluxlow-density lipoprotein particle remodelingvery-low-density lipoprotein particle assemblylow-density lipoprotein particle clearancelipoprotein metabolic processlipoprotein biosynthetic processlipoprotein catabolic processcholesterol homeostasislipoprotein transportcellular protein catabolic processregulation of cholesterol biosynthetic processartery morphogenesisleukocyte migrationcellular response to tumor necrosis factorcellular response to prostaglandin stimuluslipid metabolic processtransportlipid transportsteroid metabolic processlipid catabolic process
Process(es)
protein bindingphospholipid bindingheparin bindingcholesterol transporter activitylipase bindinglow-density lipoprotein particle receptor bindinglipid transporter activitylipid binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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